NCKU, 生理學科暨研究所

NCKU, 生理學科暨研究所

陳珮君 助理教授

陳珮君(Pei-Chun Chen)助理教授

學經歷:

長庚大學醫技系 學士,1996-2000

長庚大學 基醫所博士,2000-2006

美國芝加哥大學 博士後研究員,2006-2007

美國威斯康辛大學-麥迪遜分校 博士後研究員,2006-2007

美國奧勒岡健康和科學大學 博士後研究員,2007-2014

成大生理所 助理教授,2014-迄今

專長學科

神經退化性疾病、神經科學、分子生物學、細胞生物學、鉀離子通道胞內運輸、胰島素分泌機轉

獎勵事項

2015       “Paper of the Week” by J. Biol. Chem

2014       “Paper of the Week” by J. Biol. Chem

2013       Travel and Best Poster Awards for Postdoctoral fellows from American society of Biochemistry and Molecular Biology (ASBMB)

2011-2012   Post-doctoral Trainee, National Institute of Diabetic and Digestive and Kidney Diseases, Center Training Grant T32 DK007680

2010       Tartar Award, Oregon Health and Science University

E-mail

pcchen@mail.ncku.edu.tw

辦公室

82-0704 分機:5423

實驗室

82-0736 分機:5427

嗜好:旅遊,閱讀,陪小孩玩,看美國犯罪影集

研究方向及興趣:

主要的研究興趣是在整合週邊組織和中樞神經兩大系統來研究新陳代謝訊號如何影響到中樞神經元的活性,如何參與疾病的發生過程!

我們在研究三個鉀離子通道,分別是ATP sensitive Potassium channel (KATP channel), voltage gated potassium channel 2.1 and calcium regulated potassium channel. KATP channel這個離子通道為例, 此通道膜蛋白是會偵測細胞內ATP ADP濃度來決定通道的開啟或關閉,在胰島beta細胞裡,這個離子通道是否能正常關閉決定胰島素是否能正常釋放,在大腦的神經元細胞中,這個離子通道也參與神經元細胞的興奮與否,任何一個疾病的產生過程絕對不只是單一組織系統發生病變,細胞是否能正常執行新陳代謝的功能不只影響到自生的存亡,也會影響到中樞神經的調控,基於上述,我目前的兩個研究主題是
第一個研究主題是:在下視丘的神經細胞中,從訊號傳遞和細胞生物學的角度研究leptin怎麼調控KATP channel的活性,和一旦失調後如何參與肥胖症及糖尿病的發生。
第二個研究主題:帕金森氏症主要是因為腦中多巴胺神經元的退化,不正常的新陳代謝速度將加速細胞的死亡,利用帕金森氏症動物模式,研究KATP  Kv2.1 channels在大腦基底核神經迴路扮演的角色。

研究方法包括:

l   Protein trafficking, ion channel regulation and islet cell biology

l   Electrophysiology, cell and molecular biology techniques

l   Rodent experiments: stereotaxic microinjection of 6-OH-DA, subchronic MPTP model, behavioral assessments

實驗室成員:

現在成員

博士班:阮瀞萱(博一)

碩士班:趙如意(碩二)、 林玠寬鄭嘉惠、郭宜盈(碩一)

過去成員

碩士: 洪桂明(2016)

相關著作(PubMed連結

1.    Y. Wu, S.L. Shyng, P.C. Chen (2015), Concerted Trafficking Regulation of Kv2.1 and KATP Channels by Leptin in Pancreatic β-CellsJ Biol Chem, 290:29676-29690. "Paper of the Week”, J Biol Chem podcast on Dec 11, 2015.

2.    Q Zhou, P.C. Chen, P.K. Devaraneni, G.M. Martin, E.M Olson, S.L. Shyng, (2014) Carbamazepine inhibits ATP-sensitive potassium channel activity by disrupting channel response to MgADP. Channels, 8:376-382

3.   P.C. Chen, Y. Kryukova, S.L. Shyng, (2013), Leptin regulates KATP channel trafficking in pancreatic b-cells by a signaling mechanism involving AMPK and PKA to the plasma membrane. J Biol Chem 288:34098-34109. "Paper of the Week." And A figure from the paper was selected for the Nov. 22 cover of JBC

4.     G. Martin, P.C. Chen, P. Devaraneni, S.L. Shyng, (2013), Pharmacological rescue of trafficking-impaired ATP-sensitive potassium channels. Front Physiol. Dec 24;4:386. eCollection.

5.    P.C. Chen , E. Olson, Q. Zhou , Y. Kryukova , H. Sampson ,D.Y. Thomas, S.L. Shyng (2013), Carbamazepine as a Novel Small Molecule Corrector of Trafficking-Impaired ATP-Sensitive Potassium Channels Identified in Congenital Hyperinsulinism, J Biol Chem 288:20942-20954.

6.     H.M. Sampson, H. Lam, P.C. Chen, D. Zhang, C. Mottillo, M. Mirza, K. Qasim, A. Shrier, S.L. Shyng, J.W. Hanrahan, D.Y. Thomas (2013), Compounds that correct F508del-CFTR trafficking can also correct other protein trafficking diseases: an in vitro study using cell lines, Orphanet  J Rare Dis. 8:11.

7.     C. Escartin, S. Joon Won, C. Malgorn, G. Auregan G, A.E. Berman , P.C. Chen, N. Déglon, J.A. Johnson,S. Won Suh, R.A.  Swanson (2011), Nuclear factor erythroid 2-related factor 2 facilitates neuronal glutathione synthesis by upregulating neuronal excitatory amino Acid transporter 3 expression, J Neurosci. 31:7392-7401.

8.     P.C. Chen, C.E. Bruederle, H.Y. Gaisano, S.L. Shyng (2011), Syntaxin 1A regulates processing and endocytic trafficking of pancreatic ATP-sensitive potassium Channels, Am. J. Physiol. Cell Physiol. 300: C506-C516.

9.    F.F. Yan, E.B. Pratt, P.C. Chen, F. Wang, W.R. Skach, L.L. David, S.L. Shyng (2010), Role of HSP90 in  biognenesis of the β-CELL ATP-sensitive potassium channel complex, Mol. Biol. Cell. 21: 1945-1954. PMID: 20427569

10.   P.C. Chen, C.L. Lao and J.C. Chen (2009), The Involvement of Cdk5 signaling in D3 dopamine receptor- mediated autoinhibition in PC-12/hD3 cells, J. Neurochem. 110: 1180-1190. PMID: 19522735

11.   P.C. Chen, M.R. Vargas, A.K. Pani, R.J. Smeyne, D.A. Johnson, Y.W. Kan, and J.A. Johnson (2009), Nrf2-mediated neuroprotection in the MPTP mouse model of Parkinson’s disease: Critical role for the astrocyte, Proc. Natl. Acad. Sci. USA. 106: 2933-2938. PMID: 19196989

12.    J.A. Johnson, D.A. Johnson, A.D. Kraft, M.J. Calkins, R.J. Jakel, M.R.Vargas, P.C. Chen (2009), The Nrf2-ARE pathway: an indicator and modulator of oxidative stress in neurodegeneration, Ann N Y Acad Sci. 1147:61-9. PMID: 19076431

13.   J.C. Chen, P.C. Chen and Y.C. Chiang (2009) Molecular mechanism of psychostimulant addiction, Chang Gung Med. J. 32:148-154. PMID: 19403004

14.   P.C. Chen, C.L. Lao and J.C. Chen (2006) Dual Alteration of limbic dopamine D1 receptor-mediated signaling and Akt/GSK3 pathway in dopamine D3 receptor mutants during the development of methamphetamine sensitization, J. Neurochem. 100: 225-241. PMID: 17101033

15.  J.C.Chen, W.Z. Li, P.C. Chen, C.P. Tseng and Y.K. Huang (2006) Rat NPFF1 receptor-mediated signaling: functional comparison of neuropeptide FF (NPFF), FMRFamide and PFR(tic)amide, Peptides, 27:1005-1014. PMID: 16490283

16.    P.C. Chen and J.C. Chen (2005) Up-regulation of Cdk5 signaling in the ventral striatum of acute and chronic methamphetamine-treated rats, Neuropsychopharmacology 30: 538-549. PMID: 15536496

17.    J.C. Chen, P.C. Chen and Y.C. Chiang (2003) Dopamine D3 receptor down-regulation and functional desensitization in the olfactory tubercle of amphetamine-sensitized rats, Brain Res., 972: 159-167. PMID: 12711089 

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